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Proteins and Peptides

rMouse NGF beta Protein

Product Sizes
100 ug
£680.00
010-001-W03-0100-100UG
About this Product
SKU:
010-001-W03-0100
Additional Names:
beta-NGF|Ngf
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Nerve Growth Factor (NGF-B Beta) is a neurotrophic factor related to BDNF, NT-3 and NT-4. NGF-B Beta acts through its receptor B Beta-NGFR, and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-B Beta also is also involved in the growth, differentiation and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity. Recombinant mouse NGF-B Beta is a non-glycosylated, non-covalently linked homodimer, containing two 120 amino acid chains , with a molecular weight of 13.5 kDa each.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
Ngf
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Nerve Growth Factor beta purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Mouse Nerve Growth Factor beta Recombinant Protein. Lane 1: 1 ug Mouse NGF beta in non-reducing conditions (-). Lane 2: 1 ug Mouse NGF beta in reducing conditions (+). Lane 3: Molecular weight marker. Mouse NGF-beta is a homodimer with a predicted MW totaling 13.5 kDa.
Bioactivity of Mouse Nerve Growth Factor beta Recombinant Protein. Serial dilutions of Mouse B Beta NGF, starting at 100 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse NGF is between 0.3-0.4 ng/mL. This value is comparable to the typical expected range of less than 1 ng/mL.