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Proteins and Peptides

rMouse IL-12 Protein

Product Sizes
10 ug
£319.00
010-001-V51-0010-10UG
About this Product
SKU:
010-001-V51-0010
Additional Names:
Il12a/Il12b|NKSF, CLMF
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 su
Immunogen:
Il12a/Il12b
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Interleukin-12 purity was determined to be greater than 95% as determined by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Mouse Interleukin-12 Recombinant Protein. Lane 1: 1 ug Mouse IL-12 in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 ug Mouse IL-12 in reducing conditions (+). Mouse IL-12 is a heterodimer comprised of one 40 kDa subunit and one 35 kDa subunit. The total predicted MW is 70 kDa.
Bioactivity of Mouse Interleukin-12 Recombinant Protein. Serial dilutions of Mouse IL-12 (starting at 33 ng/mL) were added to mouse splenic T cells. After 72 hours, cells were stained to analyze surface CD8 and CD25. The linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse IL-12 is between 1-1.8 ng/mL. This value is comparable to the typically expected to be less than 2 ng/mL.