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Proteins and Peptides

rMouse FGF basic Protein

Product Sizes
10 ug
£184.00
010-001-U80-0010-10UG
About this Product
SKU:
010-001-U80-0010
Additional Names:
Fgf2|Heparin-binding growth factor 2 (HBGF-2), Prostatropin, Basic fibroblast growth factor (bFGF)
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Fibroblast Growth Factors, FGFs, are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic, or FGF-1, is a critical component in keeping human embryonic stem cells undifferentiated in cell culture systems. Recombinant mouse FGF basic is a non-glycosylated protein, containing 145 amino acids, with a molecular weight of 16.3 kDa.
Immunogen:
Fgf2
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Fibroblast Growth Factor basic purity was determined to be greater than 95% as determined by analysis of reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Mouse Fibroblast Growth Factor basic Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Mouse FGF-basic in non-reducing conditions (-). Lane 3: Molecular weight marker. Lane 4: 1 ug Mouse FGF-basic in reducing conditions (+). Mouse FGF-basic has a predicted MW of 17.2 kDa.
Mouse Fibroblast Growth Factor basic Recombinant Protein. Serial dilutions of Mouse FGF Basic, starting at 5 ng/mL, were added to NIH 3T3 cells. Cell proliferation was measured after 41 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse FGF Basic is 0.1-0.16 ng/mL. This value is comparable with the typical expected range of < 1 ng/mL.