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Proteins and Peptides

rMouse EGF Protein

Product Sizes
1 mg
£421.00
010-001-C26-1000-1MG
About this Product
SKU:
010-001-C26-1000
Additional Names:
Egf|Urogastrone, URG
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.01 M Sodium Phosphate
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4. Recombinant mouse EGF is a non-glycosylated protein, containing 54 amino acids, including 3 intra-molecular disulfide-bonds, with a molecular weight of 6.2 kDa.
Formulation:
0.01 M Sodium Phosphate, pH 7.5
Immunogen:
Egf
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Epidermal Growth Factor purity was determined to be greater than 97% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Bioactivity of Mouse Epidermal Growth Factor Recombinant Protein. 3T3 cells were cultured with 0 to 1 ng/mL Mouse EGF. Cell proliferation was measured after 44 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse EGF is 30-40 pg/mL. This value is comparable to the typical expected range less than 100 pg/mL.
SDS-PAGE of Mouse Epidermal Growth Factor Recombinant Protein. Lane 1: 1 ug Mouse EGF in non-reducing conditions (-). Lane 2: 1 ug Mouse EGF in reducing conditions (+). Lane 3: Molecular weight marker. Mouse EGF is predicted to be a homodimer with a MW of 12.4 kDa.