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Antibodies

Mouse IgA Kappa isotype Control

Product Sizes
1 mg
010-001-341-1MG
About this Product
SKU:
010-001-341
Additional Names:
Mouse IgA istoype control, Mouse IgA subclass istoype control, Mouse IgA Kappa
Application:
ELISA, FLISA, Flow Cytometry, Immunofluorescence, SDS-PAGE, Western Blot
Buffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
MAK
Concentration:
1.010 mg/ml
Extra Details:
Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.
Formulation:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype:
IgA, IgA, kappa
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Mouse IgA Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes size exclusion chromatography and antibody affinity chromatography. Analysis by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
IgG infusion in Aid-/- mice ameliorates the exacerbated HFDIO and promotes Treg cells in adipose tissue. Six-week-old male Aid+/+ and Aid-/- mice were fed with HFD for 13 weeks and received purified mouse IgG (p/n D609-0100), IgA (p/n 010-001-341) or PBS by i.v. injection during the first two and last two weeks of the diet. (a) Depiction of the study design. (b) Proportional body weight change post-injection compared with starting body weight. (c-e) Proportion of total body fat assessed by Micro-CT (c) and weight of inguinal adipose tissue (d) and epididymal adipose tissue (e). (f, g) In vivo glucose (f) and insulin (g) tolerance responses from IgG-, IgA- or PBS-infused mice. Proportion of infiltrating Treg cells in the visceral fat, identified by flow cytometry, gated from live, single CD4+ T cells prior to gating on FoxP3+ cells (h). Data shown are pooled from two independent experiments. n = 6-8 per group. Data were assessed for significance using Student's t test (c-e, h) or two-way ANOVA (b, f, g). Data are presented as mean +/- SD. *p<0.05, **p<0.01, ***p<0.001. W, week. Fig. 7. PMID: 35587276.
SDS PAGE Results of Mouse IgA kappa Isotype Control. Lane 1: Mouse IgA kappa Isotype Control Reduced [1ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Mouse IgA kappa Isotype Control Non-Reduced [1ug]. 4-20% Gel, Coomassie Stained.