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Antibodies

Mouse IgM Kappa isotype Control

Product Sizes
1 mg
£237.00
010-001-339-1MG
About this Product
SKU:
010-001-339
Additional Names:
Mouse immunoglobulin M kappa, Mouse IgM isotype control, mouse IgM kappa
Application:
ELISA, FLISA, Flow Cytometry, Immunofluorescence, SDS-PAGE, Western Blot
Buffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
MMK
Concentration:
1 mg/ml
Extra Details:
Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.
Formulation:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype:
IgM, IgM, kappa
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). MW: 5 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). Lane 2: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 3: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa Myeloma Protein (p/n 010-001-033). Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.