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Antibodies

Mouse IgG1 Lambda isotype Control

Product Sizes
1 mg
£237.00
010-001-331-1MG
About this Product
SKU:
010-001-331
Additional Names:
Mouse immunoglobulin gamma lambda, Mouse IgG1 isotype control, Mouse IgG1 Lambda
Application:
Bioactivity, ELISA, FLISA, Flow Cytometry, Immunofluorescence, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
MG1L
Concentration:
1 mg/ml
Extra Details:
Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG1, IgG1, lambda
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Characterization of the anti-IgG nanobody toolbox. (a) Overview of all identified anti-IgG nanobodies. The nanobodies obtained were characterized for IgG subclass and light chain specificity, epitope location on Fab or Fc fragment, and species cross reactivity. The protein sequences of all anti-IgG nanobodies can be found in Table S1. Nb, nanobody; CDR III, complementarity-determining region III; Gp, guinea pig; Hs, human; K Kappa, K Kappa light chain; L Lambda, lambda light chain; Fab, fragment antigen-binding, Fc, fragment crystallizable. (a. not shown) (b) IgG subclass reactivity profiling of selected anti-mouse IgG nanobodies representing all identified specificity groups. The indicated IgG species were spotted on nitrocellulose strips, and the strips were blocked with 4% (wt/vol) milk in 1x PBS. Then 300 nM of the indicated tagged nanobodies were added in milk. After washing with 1x PBS, bound nanobodies were detected using a fluorescence scanner. Note that the signal strength on polyclonal IgG depends on the relative abundance of the specific subclass (e.g., IgG2b and IgG3 are low abundance) or light chain (K Kappa/L Lambda ratio = 99:1). TP885 and TP926 showed no detectable binding to polyclonal Fab or Fc fragment and might bind to the hinge region. (p/n 010-001-331 Mouse IgG1 L Lambda lambda, 010-0105 Mouse Fab fragment). Fig 1. PMID: 29263082.
SDS-PAGE of Mouse IgG1 Lambda Isotype Control. Lane 1: Mouse IgG1 Lambda Isotype Control, Reduced. M: Opal Pre-stained Ladder (p/n MB-210-0500). Lane 2: Mouse IgG1 Lambda Isotype Control, Non-reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.