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Proteins and Peptides

rHuman GM-CSF Protein

Product Sizes
100 ug
009-F01-V11-0100-100UG
About this Product
SKU:
009-F01-V11-0100
Additional Names:
CSF2|Molgramostin, Pluripoietin-A Alpha, MGI1GM, Sargramostim
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.01 M Sodium Phosphate
CE/IVD:
RUO
Extra Details:
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. Recombinant human GM-CSF is a non-glycosylated protein, containing 128 amino acids, with a molecular weight of 14.6 kDa.
Formulation:
0.01 M Sodium Phosphate, pH 7.5
Immunogen:
CSF2
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Granulocyte Macrophage Colony Stimulating Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Granulocyte Macrophage Colony Stimulating Factor Animal Free Recombinant Protein. Lane 1: 1 ug Human GM-CSF AF in reducing conditions (+). Lane 2: 1 ug Human GM-CSF AF in non-reducing conditions (-). Lane 3: Molecular weight marker. Human GM-CSF AF is predicted to have a MW of 14.5 kDa.
Bioactivity of Human Granulocyte Macrophage Colony Stimulating Factor Animal Free Recombinant Protein. Serial dilutions of Human GM-CSF AF (starting at 5000 pg/mL) were added to TF1 cells. After 48 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human GM-CSF AF is between 0.08-0.12 ng/mL. This value is comparable to the typical expected range of 0.1-2 ng/mL.