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Proteins and Peptides

rHuman EGF Protein

Product Sizes
100 ug
£140.00
009-F01-C26-0100-100UG
About this Product
SKU:
009-F01-C26-0100
Additional Names:
EGF|Urogastrone, URG
Application:
Cell-based/Functional Assay
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4. Recombinant human EGF is a single, non-glycosylated protein containing 53 amino acids, with a molecular weight of 6.2 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
EGF
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Epidermal Growth Factor (EDF) is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by HPLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Bioactivity of Human Epidermal Growth Factor Recombinant Protein. 3T3 cells were cultured with 0 to 1 ng/mL Human EGF. Cell proliferation was measured after 44 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human EGF is 8.8-13.2 pg/mL. This value is comparable to the typical expected range of 20-100 pg/mL.