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Proteins and Peptides

rHuman VEGF-165 Protein

Product Sizes
100 ug
£898.00
009-F01-B99-0100-100UG
About this Product
SKU:
009-F01-B99-0100
Additional Names:
VEGF-A, glioma-derived endothelial cell mitogen, Vascular permeability factor (VPF)|VEGFA
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Recombinant human VEGF 165 is a non-glycosylated homodimer, containing two 165 amino acids, with a total molecular weight of 38.2 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
VEGFA
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Vascular Endothelial Growth Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by HPLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Bioactivity of Human Vascular Endothelial Growth Factor-165 Animal Free Recombinant Bioactivity of Protein. Serial dilutions of Human VEGF165 AF, starting at 50 ng/mL, were added to HUVECs. After 24 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human VEGF165 AFis 1.8-2.6 ng/mL. This value is comparable to the typical expected range of 1-6 ng/mL.