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Human Transferrin Biotin

Product Sizes
1 mg
£174.00
009-0634-1MG
About this Product
SKU:
009-0634
Additional Names:
Human Transferrin Biotin Conjugation
Application:
Dot Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
This product was prepared from normal serum by a multi-stage process that includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Transferrin and anti-Human Serum.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Ex vivo fluorescence imaging of biodistribution of the nanoparticles in an orthotopic mouse model of PBT. Notes: (A) Fluorescent signal observed in the brains of four separate mice with PBT (1-24 hours) injected intravenously (via tail vein) with transferrin-coated Prussian blue nanoparticles (MnPB-ATxRd-Tf). A control mouse with PBT was not injected with nanoparticles. (B) Representative ex vivo fluorescence imaging of organ biodistribution of the nanoparticles at 3 hours post-injection. (C) Histograms quantifying the observed fluorescence biodistribution of the nanoparticles 3 hours post-injection. ROIs for intensity measurements are indicated by white dashed lines in (B). Abbreviations: PBT, pediatric brain tumor; MnPB, manganese-containing Prussian blue; ATxRd, Texas Red-labeled avidin; Tf, transferrin; ROI, region of interest; au, arbitrary units. Human Transferrin Biotin Conjugated (p/n 009-0634). Figure 8. PMID: 24920896.
Dot Blot of Biotin conjugated Human Transferrin. Antigen: Human Transferrin Biotin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 60 min at RT.
Dot Blot results of Human Transferrin Biotin Conjugated. Antigen: Human Transferrin Biotin Conjugated. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30 min at RT. Imaging System ChemiDoc, Filter used: Chemi.