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Human Albumin Biotin Conjugated

Product Sizes
1 mg
£150.00
009-0633-1MG
About this Product
SKU:
009-0633
Additional Names:
Human Albumin Biotin Conjugated
Application:
Dot Blot, ELISA
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Human albumin or serum albumin is encoded by the ALB gene and is the most abundant plasma protein in mammals. Human albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular compartments and body tissues. Human albumin also acts as a plasma carrier by non-specifically binding several hydrophobic steroid hormones and as a transport protein for hemin and fatty acids. Too much serum albumin in the body can be harmful.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
This product was prepared from normal serum delipidation a multi-stage process including selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Albumin and anti-Human Serum.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Fatty acylated EETI-II peptides bind to serum albumin. (D) Surface Plasmon Resonance (SPR) shows fatty acylated EETI-II peptides bind to human serum albumin. SPR sensor chip SA was coated with biotinylated human serum albumin and subjected to the peptides at varying concentrations (30, 10, 3.33, 1.11, 0.37, 0.123 uM) in PBST (0.05% Tween 20). Shown are measured binding responses (black) and curve fits (red) to a two-state model, assuming two preferred fatty acid binding sites on albumin. Representative data from three independent experiments are shown. Binding constant values represent mean +/- S.E.M. Figure 4. PMID: 34712919.
Dot Blot showing the detection Biotin conjugated Human Albumin. A three-fold serial dilution of Biotin conjugated Human Albumin (p/n 009-0633) starting at 200ng was spotted onto 0.45 um nitrocellulose and blocked in 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. An HRP conjugated Streptavidin (p/n S000-03) was incubated 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP.