Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Human IgG Biotin

Product Sizes
1 mg
£144.00
009-0602-1MG
About this Product
SKU:
009-0602
Additional Names:
Human IgG Biotin conjugation, Human immunoglobulin G, Vitamin H, coenzyme R
Application:
ELISA, Immunohistochemistry, Microarray, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
Biotin
Concentration:
1.040 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Human IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule Biotin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG and anti-Human Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
ELISA Results of Human IgG Whole Molecule Biotin Conjugated. Each well was coated in duplicate with 1.0 ug of Human IgG Whole Molecule Biotin Conjugate. The working dilution is 82,800. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin-HRP (p/n S000-03) and TMB substrate (p/n TMBE-1000).
Binding ability of aptamer PA#2/8[S1-58] to living cells of S. aureus. Two S. aureus isolates 96-01678 and 05-01042 as well as formaldehyde-fixed cells of S. aureus CS (Protein A-producing Cowan strain) were used for coating the microtiter plates. Cell suspensions with an OD600 = 0.7 (1) and further stepwise dilutions (1:5, 1:10, 1:30, 1:100) were applied. 100 nM of PA#2/8[S1-58] (3'Bio) were added for binding in comparison to 0.13 nM IgG (Bio) as positive control for binding to immobilised cells. The blank reaction represents the assay control without any cell coating. Negative controls include reactions with the library (3'Bio) and without biotinylated aptamer or IgG. The results of 2 separate experiments were averaged, whereby each of them was made with 1 (IgG) or 2 (aptamer) replicates of each specific interaction. All experiments contained 1 control reaction per cell type with the library and 3 control reactions per cell type without aptamer or IgG binding reagent. Figure 6. PMID: 27650576.
Evaluation of binding ability of aptamer PA#2/8 and PA#2/8[S1-58] to bacterial cells of S. aureus by ELONA. Formaldehyde-fixed cells of S. aureus CS (Protein A-producing Cowan strain) and S. aureus WS (Protein A-deficient Wood46 Strain) as well as living cells of E. coli K12 as negative control were used for coating the microtiter plates. Cell suspensions with an OD600 = 0.7 (1) and further stepwise dilutions (1:5, 1:10, 1:30, 1:100, corresponding to OD600 = 0.14, 0.07, 0.023, 0.007) were applied. 100 nM of each biotinylated aptamer variants was added, respectively, for binding. The blank reaction represents the assay control without any cell coating. A positive control for binding to immobilised cells is represented by adding of 0.13 nM biotinylated IgG. Negative controls include reactions with the library (3'Bio), non-functional aptamer variant PA#2/8[S1-50] (3'Bio), and without biotinylated aptamer or IgG. Averaged results are shown: 2-3 separate experiments per aptamer probe and 11 experiments with IgG, whereby each of them was made with 2-3 replicates of each specific interaction. All experiments contained 1 control reaction per cell type with the library and 3 control reactions per cell type without aptamer probes or IgG. Figure 5. PMID: 27650576.