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Human Transferrin Peroxidase

Product Sizes
1 mg
£181.00
009-0334-1MG
About this Product
SKU:
009-0334
Additional Names:
Human transferrin peroxidase conjugation, HRP conjugated transferrin|TF
Application:
Dot Blot, Electron Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen:
TF
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Human Transferrin Peroxidase conjugated was prepared from normal serum by a multi-step process including selective precipitation and tandem chromatography followed by extensive dialysis against the buffer stated above. Human Transferrin Peroxidase conjugated was assayed by immunoelectrophoresis and resulted in a single precipitin arc against anti-Peroxidase, anti-Human Transferrin and anti-Human S
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
FluoroFinder:
The figure illustrates that EE tubules, defined by the presence of internalized transferrin, interact with LDs in mammalian cells. BHK cells were cotransfected with a plasmid encoding human transferrin receptor (TfR plus plasmid encoding GFP) and then incubated with transferrin-HRP for 30 min at 37°C. Cells were then processed for HRP detection and processed for electron microscopy using a mild fixation/low membrane-contrast staining method to optimize transferrin-HRP visualization. Transferrin-HRP-labeled EE tubules (arrows) were specifically associated with LDs (asterisks) as shown in the low magnification overview (left panel) and at higher magnification in the lower right panel. The two pseudocolored panels show higher magnification views of the neighboring panels, with ER in blue and the LD monolayer in orange; note the tripartite interaction with the EE tubule (EE, arrows). PMID: 32404335.
Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.
CEMM disruption releases VAMP3 from CEMMs at recycling endosomal membrane. Used a recycling endosome ablation approach by using combined treatment with 3,3'-diaminobenzidine (DAB) and H2O2 to the cells pre-loaded with horseradish peroxidase-transferrin (HRP-TF).60,61 The significant reduction of the RAB11 signaling in the recycling endosome-ablated cells (RE ablation) proved the ablation efficiency of this method (Figure 3A). Importantly, CEMM disruption-induced autophagic flux is almost totally blocked by RE ablation (Figures 3B and 3C), indicating the importance of recycling endosomes in CEMM disruption-induced autophagosome formation (A) After ablation of recycling endosomes, HeLa cells were immunostained with Rab11 (red), and observed under a confocal microscope (x600). Scale bars, 5 um. (B) After ablation of recycling endosome, HeLa cells with stable expression of GFP-LC3B were pre-treated with MBCD (5 mM, 1 h) and then incubated in the presence or absence of Baf A1 (100 nM). Then cells were observed under a confocal microscope (x600). Scale bars, 5 um. (C) The number of GFP-LC3 puncta observed in (B) are presented as means +/- SD. ****p < 0.0001. Figure 3. PMID: 34786475.