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Antibodies

Human IgA

Product Sizes
2 mg
£ POA
009-0106-2MG
About this Product
SKU:
009-0106
Additional Names:
Human Immunoglobulin A
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1.48 mg/ml
Extra Details:
Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75% of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgA
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Human IgA whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Human IgA whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific).
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Identification of b12A2 antibody secreted in the milk of transgenic mice using western blotLanes 1-8 are milk samples collected from individual mice; lane 9 is the molecular weight marker; lane 10 is milk from a negative control mouse; lane 11 is a known concentration of standard human IgA (p/n 009-0106) at 80 ng/lane; lane 12 is negative control mouse milk spiked with standard human IgA. 80 ng of IgA standard is equivalent to 0.8 mg/ml in milk when loaded at 0.1 ul of milk per lane. Reactive bands were detected using biotinylated goat anti-human IgA alpha chain (p/n 609-1606) followed by HRP conjugated streptavidin-IR800CW. Figure 1. PMID: 23269241.
SDS-PAGE of Human IgA Whole Molecule. Lane 1: Human IgA Whole Molecule, Reduced [5.0ug]. Lane 2: Human IgA Whole Molecule, Reduced [1.0ug]. Lane 3: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 4: Human IgA Whole Molecule, Non-Reduced [1.0ug]. Lane 5: Human IgA Whole Molecule, Non-Reduced [5.0ug]. 4-20% Gel, Coomassie Blue Stained. Expected MW: ~160kDa Non-Reduced, ~55kDa, and 23kDa Reduced. Additional bands: monomer, dimer and trimer.
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