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Antibodies

Human IgG F(ab')2

Product Sizes
2 mg
£246.00
009-0104-2MG
About this Product
SKU:
009-0104
Additional Names:
Human Immunoglobulin G F(ab')2 Fragment, IgG Fab2
Application:
Bioactivity, ELISA, Flow Cytometry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Human IgG F(ab')2 molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 molecules.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Human IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(ab')2
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Dose dependent inhibition curves generated with six different FcG GammaR assays. Four different set of samples were tested to show the specificity and subclass specific binding. Analytes tested are (1) human IgG subclasses IgG1, IgG2, IgG3, IgG4; (2) human IgG; (3) Fc, Fab, and F(ab)2 domains; and (4) human serum albumin (HSA). Data represent the mean +/- standard error of triplicate experiments. IC50 (nM) values calculated from the inhibition curves are shown in the Table. IC50 values are in nM. *For FcG GammaRIIb IC50 values are intended only for qualitative purposes as mentioned in the text. n.d. not determined. Figure 3. PMID: 35842448.
Anti-hinge antibodies interfere with the detection of anti-drug antibodies. A total of 115 samples from patients with no previous adalimumab exposure (A) and 91 samples from patients treated with adalimumab (B) were evaluated by flow cytometry and resulted in high anti-adalimumab MFIs. The addition of 20 ug/ml of human IgG F(ab')2 reduced the anti-adalimumab MFI signals in both naive and adalimumab-treated patients. (C) There was no difference between adalimumab-naive vs. treated groups in MFI fold-change or proportion of patients positive for anti-hinge antibodies. Anti-hinge-positive status was defined as ≥1.5-fold change as shown by the horizontal line. Fig. 3. PMID: 33008870.
SDS-Page of Human IgG F(ab')2 Fragment. Lane 1: Human F(ab')2 - Non-Reduced. Lane 2: Human F(ab')2 - Reduced. Load: 1.0 ug per lane. Predicted/observed size: 25 kDa - Reduced, 120 kDa - Non-Reduced for F(ab')2 fragment. Other band(s): None.