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Antibodies

Human IgG Fc

Product Sizes
1 mg
£266.00
009-0103-1MG
About this Product
SKU:
009-0103
Additional Names:
Human Immunoglobulin G F(c) Fragment, IgG Fc
Application:
Cell-based/Functional Assay, Dot Blot, ELISA, Immunofluorescence, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1.029 mg/ml
Extra Details:
Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No r
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-PAGE results of Human IgG F(c) Fragment. Lane 1: reduced Human IgG F(c) Fragment. Lane 2: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 3: non-reduced Human IgG F(c) Fragment. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.
TRAIL-mediated apoptosis of human plasma cells. Top, Control CESS cells (blast) or IL-6-differentiated plasma cells were cultured for 24 h in the presence or absence of 1.0 ug/ml TRAIL, DR5-Fc, or the Fc portion of human IgG. Cell viability was determined by trypan blue exclusion, and the percent of plasma cell death was calculated by dividing the number of dead plasma cells by the total number of plasma cells in the culture. Bottom, ELISA of IgG secretion in the corresponding culture medium. Data represent the relative IgG titer required to obtain a single OD value that lies within the linear range. Data represent three independent cell populations from one experiment. Three independent experiments were performed with similar results. Fig 3. PMID: 12421926.
EphB1 acts repulsive on neurons of the superficial migratory stream via reverse signaling with stripe assay. (J) After down regulation of ephrin-B3 ligands by siRNA transfection (red) in MGE-derived neurons the repulsion induced by EphB1-Fc in the stripe assay is abolished after 2 DIV, while non-transfected interneurons still avoid the EphB1-Fc stripes. (K) Application of Alexa555 labeled control siRNA has no effect as most of the cells still avoid the EphB1-Fc containing stripes. (L) Addition of ephrin-B3 siRNA does not affect MGE-derived neurons growing on Fc/control stripes. Scale bars: (J-L) 50 um. Figure 3. PMID: 25100946.
SPR interaction analyses regarding the aptamer binding site in Protein A.Biacore X100 / sensor chip CAP / ligand: biotinylated Protein A with immobilization level of ~560 RU / two-step analyte binding without regeneration in between, (A-B) analyte 1 = sample 1: human IgG, IgG-Fc fragment, IgG-Fab fragment with a concentration of 1000 nM each, or buffer, (C-D) analyte 1 = sample 1: concentration series of human IgG-Fc in the range of 0-1000 nM, (A-D) analyte 2 = sample 2: 2000 nM 5'-fluorescein-labeled aptamer PA#2/8 or buffer. Double-referenced sensorgrams are shown (blank reference surface without Protein A, buffer injection). Binding of sample 1 followed by sample 2 is shown in (A) and (C) with alignment to injection start of sample 1. In (B) and (D) only binding of sample 2 with alignment to injection start of sample 2 is shown. Fig 12. PMID: 26221730.
Western blot analysis of P19CL6_Tbx1-PA cells and P19CL6_PA control cells. Proteins were separated on 10% SDS-PAGE gel and immunoblotted with human IgG F(c). Figure 1. PMID: 19178302.