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Proteins and Peptides

rHuman Sonic Hedgehog Protein

Product Sizes
25 ug
£319.00
009-001-W24-0025-25UG
About this Product
SKU:
009-001-W24-0025
Additional Names:
HHG-1|SHH
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.01 M Sodium Phosphate
CE/IVD:
RUO
Extra Details:
Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors. Recombinant human SHH is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 19.7 kDa. The Cys at position 25 has been substituted with Ile.
Formulation:
0.01 M Sodium Phosphate, pH 7.5
Immunogen:
SHH
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Sonic Hedgehog purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
SDS-PAGE of Human Sonic Hedgehog Recombinant Protein. Lane 1: 1 ug Human SHH in non-reducing conditions (-). Lane 2: 1 ug Human SHH in reducing conditions (+). Lane 3: Molecular weight marker. Human SHH has a predicted MW of 19.7 kDa.
Bioactivity of Human Sonic Hedgehog Recombinant Protein. Serial dilutions of Human SHH, starting at 5 ug/mL, were added to with CCL-226 cells in the presence of 1 uM Retinoic Acid. Alkaline phosphatase was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human SHH is 1.2-1.8 ug/mL. The typical expected range is 0.8-1 ug/mL.