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Proteins and Peptides

rHuman SCF Protein

Product Sizes
10 ug
£319.00
009-001-W20-0010-10UG
About this Product
SKU:
009-001-W20-0010
Additional Names:
c-Kit Ligand, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor (MGF)|KITLG
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant human SCF is a non-glycosylated protein, containing 165 amino acids, with a molecular weight of 18.4 kDa
Immunogen:
KITLG
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Stem Cell Factor Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Human SCF in non-reducing conditions (-). Lane 3: Molecular weight marker. Lane 4: 1 ug Human SCF in reducing conditions (+). Human SCF has a predicted MW of 18.4 kDa.
Bioactivity of Human Stem Cell Factor Recombinant Protein. Serial dilutions of Human SCF, starting at 250 ng/mL, were added to TF-1 cells. After 68 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human SCF is 3.3-5 ng/mL. This value is comparable to the typical expected value of 1-5 ng/mL.