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Proteins and Peptides

rHuman Resistin Dimer Protein

Product Sizes
5 ug
£184.00
009-001-W19-0005-5UG
About this Product
SKU:
009-001-W19-0005
Additional Names:
Adipose tissue-specific secretory factor (ADSF), C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3|RETN
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Resistin is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Resistin is produced by adipocytes and may be an important link between obesity and insulin resistance. Recombinant human Resistin is a disulfide-linked homodimer, containing two 93 amino acid chains , with a total molecular weight of 20 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
RETN
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Resistin Dimer purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Resistin Dimer Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Human Resistin in non-reducing conditions (-). Lane 3: Molecular weight marker. Lane 4: 1 ug Human Resistin in reducing conditions (+). Human Resistin is a homodimer with a predicted total MW of 19.7 kDa.
Bioactivity of Human Resistin Dimer Recombinant Protein. 100ng/mL of SBT recombinant Human Resistin or 100 nM Insulin (+ control) was added to differentiated SH-SY5Y cells. After 10 minutes MAPK activity was measured by western blotting whole cell lysates for P-Erk and Total Erk. The figure shows that Human Resistin is able to active MAPK signaling at 100ng/mL.