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Proteins and Peptides

rHuman PDGF-AA Protein

Product Sizes
100 ug
£800.00
009-001-W08-0100-100UG
About this Product
SKU:
009-001-W08-0100
Additional Names:
PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide|PDGFA
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-A Alpha, TGF-B Beta and EGF. Recombinant human PDGF-AA is a non-glycosylated disulfide-linked homodimer, containing two 125 amino acid chains, with a total molecular weight of 28.5 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
PDGFA
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
platelet Derived Growth Factor-AA purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Platelet Derived Growth Factor-AA Recombinant Protein. Lane 1: 1 ug Human PDGF-AA in reducing conditions (+). Lane 2: 1 ug Human PDGF-AA in non-reducing conditions (-). Lane 3: Molecular weight marker. Human PDGF-AA is predicted to be a disulfide linked homodimer with a predicted MW of 28.5 kDa.
Bioactivity of Human Platelet Derived Growth Factor-AA Recombinant Protein. Serial dilutions of Human PDGF-AA, starting at 250 ng/mL, were added to 3T3 cells. Cell proliferation was measured after 46 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human PDGF-AA is 17-25 ng/mL.