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Proteins and Peptides

rHuman MIF Protein

Product Sizes
100 ug
£728.00
009-001-V79-0100-100UG
About this Product
SKU:
009-001-V79-0100
Additional Names:
Glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase|MIF
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.01 M Sodium Phosphate
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Migration Inhibitory Factor (MIF) is a pro-inflammatory cytokine that acts on fibroblasts by inducing IL-1, IL-8 and MMP expression. MIF stimulates NO production and TNF-A Alpha release following IFNG Gamma-activation of macrophages. Recombinant human MIF is a non-glycosylated protein, containing 115 amino acids, with a molecular weight of 12.5 kDa.
Formulation:
0.01 M Sodium Phosphate, pH 7.5
Immunogen:
MIF
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Migration Inhibitory Factor purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Migration Inhibitory Factor Recombinant Protein. Lane 1: 1 ug Human MIF in non-reducing conditions (-). Lane 2: 1 ug Human MIF in reducing conditions (+). Lane 3: Molecular weight marker. Human MIF has a predicted MW of 12.5 kDa.
Bioactivity of Human Migration Inhibitory Factor Recombinant Protein. Human PBMCs were cultured with 0 to 1000 ng/mL Human MIF. Production of IL-8 was measured via ELISA after 24 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human MIF is 88 - 132 ng/mL.