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Proteins and Peptides

rHuman IGF-I Protein

Product Sizes
100 ug
£319.00
009-001-V21-0100-100UG
About this Product
SKU:
009-001-V21-0100
Additional Names:
IGF1|Somatamedin C, mechano growth factor (MGF), IGF-IA
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Insulin-like Growth Factor I, IGF-I, is a growth factor produced by the liver when stimulated with growth hormone and can be found circulating throughout the body . IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100% homologous with bovine and porcine proteins. Recombinant human IGF-I is a non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.7 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
IGF1
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Insulin-like Growth Factor I purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Insulin-like Growth Factor I Recombinant Protein. Lane 1: 1 ug Human IGF-I in non-reducing conditions (-). Lane 2: 1 ug Human IGF-I in reducing conditions (+). Lane 3: Molecular weight marker. Human IGF-I has a predicted MW of 7.6 kDa.
Bioactivity of Human Insulin-like Growth Factor I Recombinant Protein. FDC-P1 cells were cultured with 0 to 250 ng/mL Human IGF-I. Cell proliferation was measured after 48 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human IGF-I is 0.65-0.98 ng/mL. This value is comparable to the competitor sample and to the expected range of less than 1 ng/mL.