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Proteins and Peptides

rHuman Growth Hormone Protein

Product Sizes
100 ug
009-001-V13-0100-100UG
About this Product
SKU:
009-001-V13-0100
Additional Names:
GH1|Growth hormone (GH), Pituitary growth hormone, Somatotropin
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Growth Hormone (GH) is an important growth factor made in and secreted throughout the body by the anterior pituitary gland. It stimulates growth and cell reproduction and regeneration in humans and other animals. The inability to appropriately produce or respond to GH results in diseases of decreased stature in children and adults. Although GH is used to treat several growth disorders, it is thought to be a very complex hormone with many of its functions yet to be uncovered. Recombinant human GH is non-glycosylated protein, containing 192 amino acids, with a molecular weight of 22.2 kDa.
Immunogen:
GH1
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Growth Hormone purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Growth Hormone Recombinant Protein. Lane 1: 1 ug Human GH in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 ug Human GH in reducing conditions (+). Lane 4: Molecular weight marker. Human GH is predicted have a MW of 22.2 kDa.
Bioactivity of Human Growth Hormone Recombinant Protein. Serial dilutions of Human GH, starting at 3 ng/mL, were added to PDF 9D11 cells. Proliferation was measured and the linear portion of the curve was us used to calculate the EC50. The ED50 of Human GH is 0.33-0.57 ng/mL. This value is comparable to the typical expected range of 0.5-0.8 ng/mL.