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Proteins and Peptides

rHuman GDF-5 Protein

Product Sizes
50 ug
£319.00
009-001-U94-0050-50UG
About this Product
SKU:
009-001-U94-0050
Additional Names:
Cartilage-derived morphogenetic protein 1|GDF5
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Growth/Differentiation Factor 5 (GDF-5) is a growth factor that regulates cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is part of the TGF family of proteins and is closely related to the BMP family of proteins. Recombinant human GDF-5 is a non-glycosylated homodimer, containing two 120 amino acids chains, with a total molecular weight of 27.4 kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
GDF5
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Growth and Differentiation Factor-5 purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Growth and Differentiation Factor-5 Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Human GDF-5 in non-reducing conditions (-). Lane 3: 1 ug Human GDF-5 in reducing conditions (+). Human GDF-5 is predicted to be a homodimer with a total MW of 26.8 kDa.
Bioactivity of Human Growth and Differentiation Factor-5 Recombinant Protein. Serial dilutions of Human GDF-5, starting at 5 ug/mL, were added to ATDC-5 cells growing in the presence of 4 ug/mL heparin. Alkaline phosphate activity was measure after 67 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human GDF-5 is 0.01 ug/mL. This value is comparable to the typical expected range of <1 ng/mL.