Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Proteins and Peptides

rHuman G-CSF Protein

Product Sizes
100 ug
009-001-U93-0100-100UG
About this Product
SKU:
009-001-U93-0100
Additional Names:
CSF-3, MGI-1G, GM-CSFB Beta, pluripoietin|CSF3
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Granulocyte-Colony Stimulating Factor (G-CSF) is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells, secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant human G-CSF is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 18.8 kDa.
Immunogen:
CSF3
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Granulocyte Colony Stimulating Factor purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Granulocyte Colony Stimulating Factor Recombinant Protein. Lane 1: 1 ug Human G-CSF in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 ug Human G-CSF in reducing conditions (+). Lane 4: Molecular weight marker. Human G-CSF has a predicted MW of 18.7 kDa.
Bioactivity of Human Granulocyte Colony Stimulating Factor Recombinant Protein. Serial dilutions of Human G-CSF, starting at 10 ng/mL, were added to NFS-60 cells. After 69 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human G-CSF is 15-22 pg/mL. This value is comparable to the typical expected 10-60 pg/mL.