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Proteins and Peptides

rHuman globular ACRP-30 Protein

Product Sizes
5 ug
£184.00
009-001-U90-0005-5UG
About this Product
SKU:
009-001-U90-0005
Additional Names:
Adiponectin, Adipose most abundant gene transcript 1 protein (apm-1), Adipocyte complement-related 30 kDa protein (ACRP30), adipolean, Gelatin-binding protein|ADIPOQ
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Globular ACRP-30, gACRP-30, is a naturally occurring cleavage product of adiponectin, a molecule made exclusively by adipocytes. gACRP-30 is detected a relatively high concentrations in the serum and is thought to play an important role in hyperglycemia and insulin resistance. gACRP-30 signals through receptors, AdipoR1 and AdipoR2. Recombinant human gACRP-30 is a non-glycosylated protein, containing 145 amino acids, with a molecular weight of 16.7 kDa.
Immunogen:
ADIPOQ
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
globular AC-30 purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human globular ACRP-30 Recombinant Protein. Lane 1: 1 ug Human gACRP-30 in non-reducing conditions (-). Lane 2: 1 ug Human gACRP-30 in reducing conditions (+). Lane 3: Molecular weight marker. Human gACRP-30 has a predicted MW of 16.7 kDa.
Bioactivity of Human globular ACRP-30 Recombinant Protein. Serial dilutions of Human gACRP-30, starting at 25 ug/mL, were added to M1 cells. After 66 hours, cell viability was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human gACRP-30 is 0.36-0.54 ug/mL. This value is comparable to the typical expected range of 1-2.5 ug/mL.