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Proteins and Peptides

rHuman FGF 147 basic Protein

Product Sizes
50 ug
£319.00
009-001-U82-0050-50UG
About this Product
SKU:
009-001-U82-0050
Additional Names:
FGF2|Heparin-binding growth factor 2 (HBGF-2), Prostatropin, Basic fibroblast growth factor (bFGF)
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154), also called FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.5 kDa.
Immunogen:
FGF2
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Fibroblast Growth Factor 147 basic purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Fibroblast Growth Factor 147 basic Recombinant Protein. Lane 1: 1 ug Human FGF147-basic in reducing conditions (+). Lane 2: 1 ug Human FGF147-basic in non-reducing conditions (-). Lane 3: Molecular weight marker. Human FGF147-basic has a predicted MW of 16.5 kDa.
Bioactivity of Human Fibroblast Growth Factor 147 basic Recombinant Protein. 3T3 cells were cultured with 0 to 10 ng/mL Human FGF basic. Cell proliferation was measured after 41 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human FGF basic is 0.1-1.4 ng/mL.