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Proteins and Peptides

rHuman FGF acidic Protein

Product Sizes
10 ug
£184.00
009-001-U79-0010-10UG
About this Product
SKU:
009-001-U79-0010
Additional Names:
FGF1|Heparin-binding growth factor 1 (HBGF-1), Beta-endothelial growth factor, ECGF-beta, acidic fibroblast growth factor (aFGF)
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-acidic, or FGF-1, is a particularly potent inducer of DNA synthesis and has chemotactic activities. Recombinant human FGF acidic is a non-glycosylated protein, containing 141 amino acids, with a molecular weight of 16 kDa.
Immunogen:
FGF1
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Fibroblast Growth Factor acidic purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Fibroblast Growth Factor acidic Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Human FGF acidic in non-reducing conditions (-). Lane 3: Molecular weight marker. Lane 4: 1 ug Human FGF acidic in reducing conditions (+). Human FGF acidic has a predicted MW of 15.8 kDa.
Bioactivity of Human Fibroblast Growth Factor acidic Recombinant Protein. Serial dilutions of Human FGF Acidic, starting at 10 ng/mL, were added to 3T3 cells in the presence of 10 ug/mL heparin. Cell proliferation was measured after 44 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human FGF Acidic is 0.8-0.12 ng/mL. This value is comparable with the typical expected range of < 1 ng/mL.