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Proteins and Peptides

rHuman FGF-4 Protein

Product Sizes
100 ug
009-001-U76-0100-100UG
About this Product
SKU:
009-001-U76-0100
Additional Names:
FGF4|Transforming protein KS3, Heparin-binding growth factor (HBGF-4), Heparin secretory-transforming protein 1 (HST-1)
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Fibroblast Growth Factor 4 (FGF-4) is a growth factor predominantly expressed during embryonic development, playing a key role in limb development. In culture, FGF-4 has been shown to be an important regulator of growth for stem cells, fibroblasts and endothelial cells. Unglycosylated FGF-4 is N-terminally cleaved into 13 kDa or 15 kDa proteins that are more active than the precursor 19 kDa protein, Bellosa P, et al. (1993). Human FGF-4 shares high homology and cross-reactivity with the mouse protein. Recombinant human FGF-4 is a non-glycosylated protein containing 140 amino acids, with a total molecular weight of 15 kDa.
Immunogen:
FGF4
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Fibroblast Growth Factor-4 purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human Fibroblast Growth Factor-4 Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Human FGF-4 in non-reducing conditions (-). Lane 3: Molecular weight marker. Lane 4: 1 ug Human FGF-4 in reducing conditions (+). Human FGF-4 is predicted have a MW of 19 kDa.
Bioactivity of Human Fibroblast Growth Factor-4 Recombinant Protein. 3T3 cells were cultured with 0 to 1 ug/mL Human FGF-4. Cell proliferation was measured after 42 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human FGF-4 is less than 0.1 ng/mL. This value is comparable to the competitor sample and to the expected range of 0.25-1.25 ng/mL.