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Proteins and Peptides

rHuman CD40 Ligand Protein

Product Sizes
10 ug
£184.00
009-001-U68-0010-10UG
About this Product
SKU:
009-001-U68-0010
Additional Names:
CD40LG|TNFSF5, TRAP, CD154, gp39, T-BAM
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.01 M Sodium Phosphate
CE/IVD:
RUO
Extra Details:
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-A Alpha, and IL-2. Recombinant human CD40 Ligand is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 16.3 kDa.
Formulation:
0.01 M Sodium Phosphate, pH 7.5
Immunogen:
CD40LG
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
CD40 Ligand purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Human CD40 Ligand Recombinant Protein. Lane 1: 1 ug Human CD40 Ligand in non-reducing conditions (-). Lane 2: 1 ug Human CD40 Ligand in reducing conditions (+). Lane 3: Molecular weight marker. Human CD40 Ligand has a predicted MW of 16.3 kDa.
Bioactivity of Human CD40 Ligand Recombinant Protein. Serial dilutions of Human CD40 Ligand, starting at 2000 ng/mL, were added to human PBMCs. After 48 hours, cell supernatant was collected and human IL-8 was measured via ELISA and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human CD40 Ligand is 50-75 ng/mL.