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Proteins and Peptides

Human Collagen Type IV

Product Sizes
500 ug
£572.00
009-001-106-500UG
About this Product
SKU:
009-001-106
Additional Names:
COL4A1-COL4A6|Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.5 M Acetic Acid
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.
Formulation:
0.5 M Acetic Acid
Immunogen:
COL4A1-COL4A6
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
This product has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.
Storage Conditions:
2-8[o]C diluted.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Native
Knockdown of NORAD inhibits proliferation, inflammation and fibrosis in HG-induced HMCs. Levels of (G) Col. IV in HMCs measured via ELISA. **P<0.01 vs. NG; ##P<0.01 vs. HG + si-NC. NORAD, non-coding RNA activated by DNA damage; NG, normal glucose; HG, high glucose; HMCs, human mesangial cells; si, small interfering RNA; NC, negative control Col. IV, type IV collagen. Figure 2. PMID: 34194552.
SDS-PAGE Results of Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5.0ug). Lane 2: Opal Prestained Molecular Weight Protein (p/n MB-210-0500). Lane 3: Human Collagen Type IV Non-Reduced (5.0ug). 4-20% Gel, Coomassie Stained. Observed MW: Type IV A Alpha1, A Alpha2, A Alpha3 bands.
NORAD reverses the inhibitory effects of miR-485 on HG-induced HMCs. (F) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs measured via ELISA **P<0.01 vs. miR-NC; #P<0.05 vs. miR-485 mimics. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1. Figure 4. PMID: 34194552.
NORAD knockdown inhibits proliferation, inflammation and fibrosis in HG-induced HMCs by regulating the miR-485/NRF1 axis. (G) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs as determined via ELISA. **P<0.01 vs. si-NC; #P<0.05 vs. si-NORAD + inhibitor NC; &P<0.05 vs. si-NORAD + ov-NC. miR, microRNA; NORAD, non-coding RNA activated by DNA damage; NRF1, nuclear respiratory factor 1; HG, high glucose; HMCs, human mesangial cells; ov, overexpression vector; si, small interfering RNA; NC, negative control; Col. IV, type IV collagen; FN, fibronectin; PAI-1, plasminogen activator inhibitor 1. Figure 6. PMID: 34194552.