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Proteins and Peptides

Human Collagen Type I

Product Sizes
500 ug
£472.00
009-001-103-500UG
About this Product
SKU:
009-001-103
Additional Names:
COL1A1/COL1A2|Type I collagen, collagen 1, human collagen
Application:
Cell-based/Functional Assay, ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.5 M Acetic Acid
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Collagen type I is the fibrillar collagen found in most connective tissues, including cartilage. Collagen type I is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera).
Formulation:
0.5 M Acetic Acid
Immunogen:
COL1A1/COL1A2
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Human Collagen type I reacts with anti-Collagen Type I. Reaction of Human Collagen type I with Rockland's anti-Collagen II, III, IV, V or VI is negligible.
Storage Conditions:
2-8[o]C diluted.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Native
Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
Glycated collagen 1 regulates adipocyte insulin signaling and Rho and AGE scavenger receptor expression. Preadipocytes from human VAT were differentiated into mature adipocytes in 2D culture +/- unglycated or glycated recombinant human collagen 1 (UC1, GC1), then studied with glucose uptake assay or RT-qPCR. (a) Glucose uptake in non-stimulated (basal) or insulin-stimulated conditions. Ordinate: mean glucose uptake measured by 3H-2D-glucose in cell lysates (cpm) normalized to cell lysate protein concentration (mg/ml); bars with different letters indicate P < 0.050; n = 15 NDM, 15 DM subjects. (b) Gene expression studied with RT-qPCR. Ordinate: mean fold difference in transcript level in GC1 arm relative to UC1 arm referent = 1; *P < 0.050, comparing transcript levels in GC1 arm vs. UC1 arm; n = 12 NDM, 12 DM subjects. Figure 2. PMID: 31875018.
SDS-PAGE Results of Human Collagen Type I. Lane 1: Human Collagen Type I Reduced (5.0ug). Lane 2: Opal PRestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Human Collagen Type I Non-Reduced (5.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type I trimer, Type I dimer A Alpha1, A Alpha2, Type I monomer A Alpha1, A Alpha2.