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Antibodies

Hamster IgG

Product Sizes
5 mg
£212.00
007-0102-0005-5MG
About this Product
SKU:
007-0102-0005
Additional Names:
Hamster Immunoglobulin Gamma, Immunoglobulin G
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
10 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, Hamster immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Hamster IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Hamster IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-GS Hamster IgG and anti-GS Hamster Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Combination therapy with Cy/GVAX and PD-1 or PD-L1 blockade improves clinical outcomes in a PDA mouse model. Anti-PD-1, anti-PD-L1 or IgG (5 mg/kg IP) were administered IP twice weekly until death starting on day 3. (B) Kaplan-Meier survival curves of mice that were implanted with PDA cells and were treated with different combinations of Cy, GVAX and the A AlphaPD-1 antibody. The percentages of mice that remained disease free at day 90 following tumor implantation and therapy with (C) Cy, GVAX and/or A AlphaPD-1 or (D) Cy, GVAX and A AlphaPD-L1 are shown. All the p values were yielded by comparing GVAX and/or A AlphaPD-1/A AlphaPD-L1 treatment groups with IgG treated group. (E) Kaplan-Meier survival curves of mice that were implanted with Panc02 cells via hemispleen technique and treated with different combinations of Cy, GVAX and A AlphaPD-L1 antibody. Data are represented as results obtained from experiments with 8-10 mice per group that were repeated at least twice. Fig 2. PMID: 25415283.
Anti-PD-1 antibody plus radiation therapy (RT) cures mice with intracranial GL261-luc tumors. (A) Experimental timeline. (B) Luciferase imaging of 4 distinct mice per treatment arm before treatment (day 7) and after treatment (day 21), divided by treatment group. All images at same scale. All mice individually matched on days 7 and 21. (C) Kaplan-Meier survival curve. P<.05 between RT+anti-PD-1 antibody arm and all other arms. All experiments repeated in triplicate with >6 mice per arm. Fig. 2. PMID: 23462419.
Anti-CD154-facilitated alloengraftment is multilineage. Twenty mice from 2 representative experiments shown in Figure 1 were phenotyped at 120 days after BMT for donor-host origin of CD4+ and CD8+ T cells, CD19+ B cells, and MAC-1+ myeloid cells. On the x-axis are shown the host and donor proportions of each of the lineages. indicates the proportion of each lineage of host origin; , the proportion of each lineage that is of donor origin. On the y-axis is shown the percentage of PBLs of each lineage. Irrelevant hIgG-treated mice had no detectable donor chimerism and thus are composed entirely of host-type cells. Note that most CD4+ T cells, CD19+ B cells, and MAC-1+ myeloid cells in anti-CD154-treated mice are of donor origin. In contrast, most of the CD8+ T cells are of host origin. Fig. 3. PMID: 11435318.
SDS-PAGE Results of Golden Syrian Hamster IgG Whole Molecule. Lane 1: Golden Syrian Hamster IgG Whole Molecule, Reduced [5.0ug]. Lane 2: Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Golden Syrian Hamster IgG Whole Molecule, Non-Reduced [5.0ug]. 4-20% gel, Coomassie stained.
Anti-CD40L mAb alone or in combination with (A) sirolimus or (B) CsA results in long-term multilineage engraftment. Fig. 1. PMID: 12384443.