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Antibodies

Goat IgG Fc

Product Sizes
2 mg
£ POA
005-0103-2MG
About this Product
SKU:
005-0103
Additional Names:
Goat IgG F(c) fragment, Goat IgG Fc fragment
Application:
SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
2.44 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(c). No reacti
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS PAGE Results of Goat IgG F(c) Fragment. Lane 1: Goat IgG F(c) Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Goat IgG F(c) Non-Reduced [1.0ug]. 4-20% Gel, Coomassie Stained.
SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n 005-0002). Lane M: 5 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n 005-0002). Lane 2: Reduced Goat IgG F(c) Fragment (p/n 005-0103). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n 005-0105). Lane 4: Reduced Goat IgM Whole Molecule (p/n 005-0107). Load: 1 ug for IgG, F(c) and F(ab); 3 ug for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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