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Antibodies

Dog IgG

Product Sizes
5 mg
£151.00
004-0102-0005-5MG
About this Product
SKU:
004-0102-0005
Additional Names:
Dog immunoglobulin G, Canine IgG
Application:
Bioactivity, Cell-based/Functional Assay, ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
10 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Dog IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Dog IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG and anti-Dog Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Full-length coagulase protein binding to prothrombin, canine IgG, and complement C3. Data were obtained from three independent experiments. (A) ELISA results showing coagulase binding of canine IgG, Fc, and Fab. There was a significant interaction of treatment (IgG, Fc, and Fab) with the binding of coagulase protein (P = 0.010). All three treatments had significant differences between results at different concentrations (P < 0.001). For IgG concentrations of 10, 5, 2.5, and 1.25 ug/ml, binding was significantly different from that of the negative control (*, P < 0.001). For Fc and Fab concentrations of 5 and 10 ug/ml, binding was significantly different from that of the negative control (*, P < 0.001). The coagulase protein bound more IgG and Fc than Fab. At 5 and 10 ug/ml, IgG binding was significantly higher than that of Fab (P = 0.008), and binding of IgG and Fc was marginally different (P = 0.054). Fig 4. PMID: 29891539.
SDS-PAGE of Dog IgG Whole Molecule. Lane 1: Dog IgG Whole Molecule Reduced (10ug). Lane 2: Dog IgG Whole Molecule Reduced (5ug). Lane 3: Dog IgG Whole Molecule Reduced (1.0ug). Lane 4: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 5: Dog IgG Whole Molecule Non-Reduced (10ug). Lane 6: Dog IgG Whole Molecule Non-Reduced (5ug). Lane 7: Dog IgG Whole Molecule Non-Reduced (1.0ug). 4-20% Gel, Coomassie Stained.