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Antibodies

Chicken IgG Alkaline Phosphatase

Product Sizes
1 mg
£234.00
003-0502-1MG
About this Product
SKU:
003-0502
Additional Names:
Chicken IgG alkaline phosphatase conjugated, Chicken IgG alk phos conjugated, Chicken IgY alkaline phosphatase conjugated, Chicken IgY alk phos conjugated
Application:
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol
CE/IVD:
RUO
Conjugate:
AP
Concentration:
1 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Chicken IgG whole molecule is ideal for investigators in Cancer, Immunology, and Microbiology research.
Formulation:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken Serum and anti-Alkaline Phosphatase (calf intestine).
Storage Conditions:
2-8[o]C unopened. Do not freeze.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane M: 5 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Reduced Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Reduced Chicken IgM Whole Molecule (p/n 003-0107). Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.