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Antibodies

Chicken IgM

Product Sizes
1 mg
£300.00
003-0107-1MG
About this Product
SKU:
003-0107
Additional Names:
Chicken Immunoglobulin M, Fowl IgM
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Formulation:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype:
IgM
Physical State:
Liquid
Purity:
>95%
Shipping Conditions:
Blue Ice
Specificity:
Chicken IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Chicken IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and anti-Chicken IgM (u chain specif
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane M: 5 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Reduced Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Reduced Chicken IgM Whole Molecule (p/n 003-0107). Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.