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Antibodies

TrueBlot[R] Anti-Mouse Ig IP Agarose Beads

Product Sizes
2.5 ml
00-8811-25-2.5ML
About this Product
SKU:
00-8811-25
Additional Names:
Anti-Mouse immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Ms IgG, Goat anti-Mouse IgG, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot.
Application:
Bioactivity, Immunoprecipitation, SDS-PAGE, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Agarose
Concentration:
14.4 mg/cc
Extra Details:
TrueBlot® Anti-Mouse Ig IP Agarose Beads are a suspension of activated agarose beads coupled with goat Anti-mouse IgG. It is suitable for precipitation of mouse IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Isotype:
IgG
Physical State:
Suspension of agarose beads
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
TrueBlot® Anti-Mouse Ig IP Agarose Beads has been tested in SDS-Page, immunoprecipitation, and western blot.
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Immunoprecipitation/Western Blot using GFP Protein. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: GFP Input (p/n 000-001-215) Reduced [10uL]. Primary IP Antibody: Mouse Anti-GFP (p/n 600-301-215) at 10ug overnight at 2-8°C. Secondary Antibody: TrueBlot Anti-Mouse Ig IP Agarose Beads (p/n 00-8811-25) at 500ug for 1hr at RT. Buffer: BlockOut Buffer (p/n MB-073) for 30 mins at RT. Exposure: 7 sec.
Loss of LIFR activates NF-K KappaB signaling through SHP1, leading to upregulation of LCN2. (a) HEK293T cells were transfected with HA-FLAG-SHP1 and SFB-tagged GFP or LIFR. LIFR-SFB protein was pulled down with S-protein beads, followed by immunoblotting with antibodies against FLAG and HA. (b) HEK293T cells were transfected with MYC-SHP2 and SFB-tagged GFP or LIFR. LIFR-SFB protein was pulled down with S-protein beads, followed by immunoblotting with antibodies against FLAG and MYC. (c) HEK293T SFB-GFP and SFB-LIFR stable cell lines were infected with the scrambled (Scr) or sh-SHP1 lentivirus, followed by transfection with a K63-specific mutant of His-Xpress-ubiquitin (Ub). 48 h later, cells were subjected to pulldown with nickel beads and immunoblotting with antibodies against TRAF6 and Xpress. (d) Control and LIFR-overexpressing PLC/PRF/5 cells were transduced with SHP1 shRNA and immunoblotted with the indicated antibodies. (e) Control (Scr) and LIFR-knockdown HEK293T cells were transfected with FLAG-TRAF6. 48 h later, cells were immunoprecipitated with a FLAG-specific antibody and immunoblotted with antibodies against LIFR, SHP1, and FLAG. Fig. 4. PMID: 34921145.
GAGE12 modulates H3K56Ac through attenuation of HDAC activity. (E) Interaction of FLAG-GAGE12, SYNM, HDAC1/2, and B Beta-actin in total nuclear lysates of mock and f:G12-OE cells. In FLAG immunoprecipitates, SYNM, HDAC1/2, and B Beta-actin are only pulled down in the presence of FLAG-GAGE12 (lanes 3 and 4). In SYMN immunoprecipitates, SYNM only pulled down HDAC1 and HDAC2 in the presence of FLAG-GAGE12. SYNM interacts with B Beta-actin, even in the absence of GAGE12 (lanes 5 versus 6). (F) Increased HDAC1-HDAC2-B Beta-actin interaction in the presence of GAGE12. In HDAC1 and HDAC2 immunoprecipitates, increased B Beta-actin was found in the pull-down lysates only in the presence of GAGE12. Figure 6. PMID: 34469741.