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Antibodies

TrueBlot[R] Anti-Rabbit Ig IP Agarose Beads

Product Sizes
2.5 ml
£247.00
00-8800-25-2.5ML
About this Product
SKU:
00-8800-25
Additional Names:
Anti-Rabbit Immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, TrueBlot for immunoprecipitation, IP beads for TrueBlot, Anti-Rabbit IgG IP agarose
Application:
ChIP, Immunoprecipitation, SDS-PAGE, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Agarose
Concentration:
8.9 mg/cc
Extra Details:
TrueBlot® Anti-Rabbit Ig IP Agarose Beads are a suspension of activated agarose beads coupled with goat Anti-rabbit IgG. It is suitable for precipitation of rabbit IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Isotype:
IgG
Physical State:
Suspension of agarose beads
Reactivities:
Rabbit
Shipping Conditions:
Blue Ice
Specificity:
TrueBlot® Anti-Rabbit Ig IP Agarose Beads have been tested in SDS-Page, immunoprecipitation, and western blot.
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of TrueBlot® Anti-Rabbit Ig IP Agarose Beads. Lane 1: Protein Standard Opal Pre-stained (p/n MB-210-0500). Lane 2: Rb-a-GFP Input. Lane 3: Rb-a-GFP Unbound. Lane 4: Rb-a-Elute. Primary Antibody: TrueBlot® Anti-Rabbit Ig IP Agarose Beads (00-8800-25) at 1 ug/mL for overnight at 4°C. Secondary Antibody: TrueBlot® Rabbit Secondary HRP Antibody (p/n 18-8816-33) at 1:10,000 for 30 min at RT. Block: MB-070 at RT for 30 min. Predicted/Observed size: 27 kDa.
ADGRF1 overexpression activates GA Alphas/GA Alphaq pathways. BT474 clone 1 and SKBR3 clone 2 were grown in absence (-) or presence (+) of doxycycline (Dox). A, Co-immunoprecipitation with anti-GA Alphas and immunoblotting with anti-HA indicate that ADGRF1 couples to GA Alphas in both BT474 and SKBR3. B, Basal levels of cAMP were significantly increased upon ADGRF1 overexpression with Dox in both cells. C, Co-immunoprecipitation with anti-GA Alphaq and immunoblotting with anti-HA indicate that ADGRF1 couples also to GA Alphaq in both cells. D, Basal levels of IP1 were significantly increased upon ADGRF1 overexpression with Dox in BT474 but not SKBR3 cells. *indicates statistically significant difference P < .05 by unpaired t test (N = 3-4). FIGURE 5. PMID: 34110646.
MLL5 interacts with CRX via its CD4 domain (A) Interaction between endogenous MLL5 and CRX. Equivalent amounts of retinal lysates from Mll5-WT or Mll5-KO mice were immunoprecipitated with anti-MLL5 antibody, anti-CRX or normal rabbit IgG, followed by western blotting detection. (B) Schematic representation of MLL5 truncated mutants. PS, PHD/SET domain; CD, central domain; CT, C-terminal domain. D DeltaCT4, deleted CT4 domain. (C) MLL5 central domain (MLL5-CD) binding to CRX. HEK293T cells expressing HA-CRX together with Flag-MLL5-PS, Flag-MLL5-CD, or Flag-MLL5-CT were subjected to immunoprecipitation with the indicated antibodies followed by western blotting detection. (D) Interaction between MLL5-CD4 and CRX. HEK293T cells expressing HA-CRX and Flag-MLL5-CD4 were immunoprecipitated with anti-HA antibodies and detected by anti-Flag and anti-HA antibodies. (E) Depletion of MLL5 CD4 compromises MLL5 interaction with CRX. HEK293T cells expressing HA-CRX along with Flag-MLL5-FL or Flag-MLL5-D DeltaCD4 were immunoprecipitated with anti-HA and anti-Flag antibodies followed by western blotting detection. (F) MLL5-D DeltaCD4 was less potent than MLL5-WT at rescuing transcription of Rho. qPCR analysis of Rho transcript in 661W (CRX OE) cells transfected with MLL5-siRNA (siMLL5) followed by transduction with the indicated lentivirus. Rho expression was normalized to the expression of Tbp. Error bars represent SEM (n = 3), **p < 0.01, Student's t test. Results in (A-F) are representative of at least three experimental repeats. Fig 4. PMID: 35359806.