Hexa-histidine (Epitope Tag) Antibody
Product Sizes
100 ug
£671.00
MSM1-3550-P1-100UG
About this Product
- SKU:
- MSM1-3550-P1
- Application:
- Flow Cytometry, Immunofluorescence, Western Blot
- translate.label.attr.clone:
- 6HIS/3550
- Clonality:
- Monoclonal
- Extra Details:
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This MAb is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds. It has a very high affinity for hexa-histidine and is excellent for use in various techniques. His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Epitope tags are useful for the labeling and detection of proteins using immunoblotting; immunoprecipitation; and immunostaining techniques. Because of their small size; they are unlikely to affect the tagged protein s biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems; this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.
- Host:
- Mouse
- Immunogen:
- Hexa-histidine tagged human CD70 recombinant protein
- Isotype:
- IgG1
- Molecular Weight:
- 244Da
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Supplier:
- NeoBiotechnologies
- Type:
- Antibodies: Monoclonal Antibody
- Manufacturer's Data Sheet:https://www.neobiotechnologies.com/download-datasheet/?PID=125581
