Maltose Binding Protein / MBP-probe Antibody
Product Sizes
20 ug
£364.00
MSM1-2847-P0-20UG
About this Product
- SKU:
- MSM1-2847-P0
- Application:
- Immunofluorescence, Western Blot
- translate.label.attr.clone:
- R29.6
- Clonality:
- Monoclonal
- Extra Details:
- Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial; insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part; eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP); the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose; the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively; the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution; MBP fusion protein can be visualized either by Western Blot Analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.
- Host:
- Mouse
- Immunogen:
- MOS maltose binding protein fusion protein.
- Isotype:
- IgG1
- Molecular Weight:
- 40kDa
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Supplier:
- NeoBiotechnologies
- Type:
- Antibodies: Monoclonal Antibody
- Manufacturer's Data Sheet:https://www.neobiotechnologies.com/download-datasheet/?PID=125551
