DNA-RNA Hybrid Antibody
Product Sizes
100 ug
£671.00
MSM1-2006-P1-100UG
About this Product
- SKU:
- MSM1-2006-P1
- Application:
- Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- translate.label.attr.clone:
- S9.6
- Clonality:
- Monoclonal
- Extra Details:
- We have not tested this antibody in-house in Immunofluorescence; CHIP; Immunocytochemistry; Immunoprecipitation or Flow Cytometry. All application recommendations come from publications using this clone. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation; with a second target for single-stranded nucleic acid binding proteins on the complementary; displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally; but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA; double-stranded DNA and RNA; and ribosomal RNA were not bound by clone S9.6 (Boguslawski; S.J.; et al. (1986). J. Immunol Methods. 89(1):123-130).
- Host:
- Mouse
- Immunogen:
- DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase
- Isotype:
- IgG2a
- Molecular Weight:
- Not Known
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Supplier:
- NeoBiotechnologies
- Type:
- Antibodies: Monoclonal Antibody
- Manufacturer's Data Sheet:https://www.neobiotechnologies.com/download-datasheet/?PID=138859
