Mouse IgE ELISA Kit
Product Sizes
1 plate
£497.00
E-90E-1PLATE
About this Product
- SKU:
- E-90E
- Application:
- ELISA
- Extra Details:
- The assay is a double antibody sandwich Mouse IgE ELISA. In this assay the IgE present in samples reacts with the anti-Mouse IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3a',5,5a'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
- Host:
- Goat
- Immunogen:
- IgE
- Physical State:
- Plasma, Serum
- Range:
- 6.25 ng/ml - 400 ng/ml
- Reactivities:
- Non-human Primate
- Sensitivity:
- 1.911 ng/ml
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8[o]C
- Supplier:
- Immunology Consultants Laboratory
- Type:
- :
