Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Alpha-cardiac actin Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
M1206-1-100UL
About this Product
SKU:
M1206-1
Application:
Western Blot, IHC-Paraffin, IHC-Frozen
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
33-32
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide within Human Alpha-cardiac actin aa 1-50 / 377.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 42 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Alpha-cardiac actin on different lysates with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1;000 dilution. Lane 1: Mouse lung tissue lysate (40 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Lane 3: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1206-1) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M1206-1) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M1206-1) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Mouse anti-Alpha-cardiac actin antibody (M1206-1) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M1206-1) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.