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Antibodies

FAM38A / PIEZO1 Mouse monoclonal Antibody

Product Sizes
50uL
£245.00
M1005-2-50UL
100μl
£400.00
M1005-2-100UL
About this Product
SKU:
M1005-2
Application:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
2-10
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human PIEZO1 aa 1275-1540 / 2521.
Isotype:
IgG2a
Molecular Weight:
Predicted band size: 287 kD
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of FAM38A / PIEZO1 on recombinant protein with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of FAM38A / PIEZO1 on MCF-7 cell lysates with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 287 kDa Observed band size: 287 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of Siha cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.