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Antibodies

Cancer Associated Fibroblast Marker Antibody Kit

Product Sizes
1 kit
£600.00
K2006-1KIT
About this Product
SKU:
K2006
Application:
Western Blot
Shipping Conditions:
Blue Ice
Supplier:
Huabio
Type:
Antibody
Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (ET1610-39) at 1/20;000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: C6 cell lysate (10 µg/Lane) Lane 6: C2C12 cell lysate (10 µg/Lane) Lane 7: RAW264.7 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: Lane 1-5: 3 seconds; Lane 6-7: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-39) at 1/20;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Fluorescence multiplex immunohistochemical analysis of the human pancreatic carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD31 (M1511-8; green); anti-α-SMA (ET1607-53; red) and anti-FAP (ET1704-23; yellow) on human pancreatic carcinoma. Panel B: anti- CD31 stained on the endothelial cells. Panel C: anti-α-SMA stained on cancer-associated fibroblasts and smooth muscle cells. Panel D: anti-FAP stained on the cancer-associated fibroblasts. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in three rounds of staining: in the order of M1511-8 (1/5000 dilution); ET1704-23 (1/1000 dilution); and ET1607-53 (1/3000 dilution) for 20 mins at room temperature. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Nikon ECLIPSE Ni-E microscope.
Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody (ET1607-53) at 1/20;000 dilution and competitor's antibody at 1/2;000 dilution. Lane 1: Saos-2 cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: C2C12 cell lysate (15 µg/Lane) Lane 5: Neuro-2a cell lysate (15 µg/Lane) Lane 6: Mouse skin tissue lysate (30 µg/Lane) Lane 7: Rat skin tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 46 seconds 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-53) at 1/20;000 dilution and competitor's antibody at 1/2;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling alpha smooth muscle Actin (ET1607-53). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (ET1607-53; red) at 1/1;000 dilution overnight at 4 ℃; washed with PBS. iFluor™ 647 conjugate-Goat anti-Rabbit IgG (HA1123) was used as the secondary antibody at 1/1;000 dilution. DAPI was used as nuclear counterstain.