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Antibodies

Neuronal Marker IF Antibody Sampler Kit

Product Sizes
1 kit
£520.00
HAK21102-1KIT
About this Product
SKU:
HAK21102
Application:
Western Blot, IHC-Paraffin, IHC-Frozen
Shipping Conditions:
Blue Ice
Supplier:
Huabio
Type:
Antibody
Fluorescence multiplex immunohistochemical analysis of mouse hippocampus (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-GFAP (ET1601-23; Green); anti-NeuN (ET1602-12; Red) and anti-c-Fos (HA722666; White) on hippocampus. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1601-23 (1/1;000 dilution); ET1602-12 (1/1;000 dilution) and HA722666 (1/200 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-GFAP (ET1601-23; Green); anti-NeuN (ET1602-12; Red) and anti-c-Fos (HA722666; White) on brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1601-23 (1/1;000 dilution); ET1602-12 (1/1;000 dilution) and HA722666 (1/200 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-MAP2 (HA500177; Red); anti-Olig2 (ET1604-29; Cyan); anti-GFAP (ET1601-23; Magenta) and anti-Neun (ET1602-12; Yellow) on mouse brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in four rounds of staining: in the order of HA500177 (1/1;000 dilution); ET1604-29 (1/5;000 dilution); ET1601-23 (1/10;000 dilution) and ET1602-12 (1/10;000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Fluorescence multiplex immunohistochemical analysis of mouse hippocampus (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-MAP2 (HA500177; Green); anti-GFAP (ET1601-23; Red) and anti-NeuN (ET1602-12; Magenta) on Mouse hippocampus. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in three rounds of staining: in the order of HA500177 (1/1;000 dilution); ET1601-23 (1/1;000 dilution) and ET1602-12 (1/10;000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.