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Antibodies

Human Reactive Exosome Marker Antibody Sampler Kit

Product Sizes
1 kit
£760.00
HAK21094-1KIT
About this Product
SKU:
HAK21094
Application:
Western Blot
Shipping Conditions:
Blue Ice
Supplier:
Huabio
Type:
Antibody
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/2;000 dilution. Lane 1: HeLa cell lysate; 20 µg/Lane Lane 2: K-562 cell lysate; 20 µg/Lane Lane 3: MCF7 cell lysate; 20 µg/Lane Lane 4: HCT 116 cell lysate; 20 µg/Lane Lane 5: HepG2 cell lysate; 20 µg/Lane Lane 6: SK-MEL-28 cell lysate; 20 µg/Lane Lane 7: A375 cell lysate; 20 µg/Lane Lane 8: B16-F1 cell lysate; 20 µg/Lane Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET1601-9; 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of TAPA1/CD81 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-87; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5;000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell lysate Lane 2: JAR cell lysate
Western blot analysis of CD63 on different lysates with Rabbit anti-CD63 antibody (HA722731) at 1/1;000 dilution. Lane 1: SK-MEL-28 cell lysate; 20 µg/Lane Lane 2: Jurkat cell lysate (negative); 20 µg/Lane Lane 3: U-87 MG cell lysate; 20 µg/Lane Lane 4: HUVEC cell lysate; 20 µg/Lane Lane 5: K-562 cell lysate; 20 µg/Lane Lane 6: HEK-293 cell lysate; 20 µg/Lane Lane 7: HL-60 cell lysate; 20 µg/Lane Predicted band size: 26 kDa Observed band size: 30-65 kDa Exposure time: Lane 1: 4 seconds; Lane 2-7: 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722731) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.