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Antibodies

Cell Fractionation Antibody Sampler Kit

Product Sizes
1 kit
£440.00
HAK21080-1KIT
About this Product
SKU:
HAK21080
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation
Shipping Conditions:
Blue Ice
Supplier:
Huabio
Type:
Antibody
Western blot analysis of MEK1/2 on different lysates with Rabbit anti-MEK1/2 antibody (ET1602-3) at 1/5;000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: A431 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-3) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of PC-12 cells labeling MEK1/2 with Rabbit anti-MEK1/2 antibody (ET1602-3) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MEK1/2 antibody (ET1602-3) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (ET1701-64) at 1/20;000 dilution and competitor's antibody at 1/5;000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: HEK-293 cell lysate Lane 5: C2C12 cell lysate Lane 6: L-929 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-64) at 1/20;000 dilution and competitor's antibody at 1/5;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3 (ET1701-64) / Competitor's antibody / Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin; which is equivalent to one.